Filters

DNA bank for cultivated rice, wild rice and related genera genetic resources

Contributors to this page: T.T. Chang Genetic Resources Centre-IRRI, Los Baños, Philippines (Ruaraidh Sackville Hamilton, Ken McNally, Flora de Guzman, Renato Reaño, Soccie Almazan, Adelaida Alcantara, Elizabeth Naredo); WARDA, Cotonou, Benin (Ines Sánchez); UPLB-University of the Philippines at Los Baños (Teresita Borromeo).

Contents:
Sample processing
Viability and monitoring
Storage

When DNA banks are used

DNA banks should be used for small core collections of accessions with the intention of repeated use for gene discovery. DNA conservation of rice germplasm is a short-term and expensive alternative. Due to difficulties of molecular characterization of heterogeneous populations, conserving materials as DNA is justifiable only for accessions with immediate value for intensive research.

The DNA bank serves as a cost-effective source for research on identical DNA samples. It does not replace conservation of the original accession as seed.

Sample processing

Taking rice leaf samples of transgenic lines that will be subjected to DNA extraction in the laboratory (photo: IRRI)

Source of material (DNA acquisition)

Type of plant material

Amount of plant material

Pre-treatments

Sample preparation

DNA extraction and purification

DNA replication/multiplication

Sample packaging

Recording information during sample processing

The following information must be recorded for each processing step:

Back to top


Viability and routine monitoring

An agricultural research technician performs a Polymerase Chain Reaction (PCR) procedure to determine the presence of alleles or genes with known function or importance (photo: IRRI)

Storage DNA quality monitoring

Type of test

Number of replicates

Frequency

Minimum number of samples

Recording information during DNA storage monitoring

The following information must be recorded for each step:

Back to top


Storage in DNA banks

Storage specifications

Storage conditions

Duration of storage

Legal arrangements

Safety duplication

Since DNA conservation is justified only as an additional measure for cost-effective support of active research, not replacing the seed sample, the concept of safety duplication does not normally arise.

Storage management in DNA banks

System for tracking material/inventory system

Recording information during DNA storage

The following information must be recorded for each step:

References and further reading

Fulton TM, Chunwongsw J, Taksley SD. 1995. Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Molecular Biology Reporter. 13(3):207-209. Available for purchase from: http://www.springerlink.com/content/x26j8q2r7644r668/. Date accessed 15 July 2013.

Back to top

The Genebanks

The 11 CGIAR genebanks currently conserve 730,000 of cereals and grain legumes, forage crops, tree species, root and tuber crops, bananas and crop wild relatives.

close-icon